Abstract Background Real-time RT-PCR has become a common and robust technique to detect and quantify low-abundance mRNA expression and is a prefered tool when examining fungal gene expression in infected host tissues. However. correct evaluation of gene expression data requires accurate and reliable normalization against a reference transcript. Thus. https://www.roneverhart.com/AMS-Neve-1073SPX-Single-Preamp-and-EQ-p33827/